纳米酶在葡萄糖分析检测中的应用研究进展

doi:10.3969/j.issn.0258-8021.2016. 01.013

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摘要即时、准确的监控血糖和尿糖的变化对于糖尿病的早期诊断及血糖控制具有重要意义。自从2004年报道金纳米簇具有磷酸转移酶样催化活性以来，多种具备酶样催化活性的纳米材料被发现，包括过氧化物酶、过氧化氢酶、超氧化物歧化酶、氧化酶等。与天然酶相比，纳米酶具有成本低廉、制备简单、易于保存运输、环境耐受性高等优点。利用纳米酶进行葡萄糖分析检测有望降低检测成本，提高检测系统稳定性。本文总结了金属纳米颗粒、金属氧化物、金属硫化物、碳基材料以及复合材料等多种纳米酶检测系统在葡萄糖分析检测中的特点、检测限和检测范围，并对纳米酶研究面临的挑战及前景进行展望。

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	罗成¹李艳¹ 龙启文²龙建纲^3*

关键词 ：纳米酶, 过氧化物酶, 葡萄糖氧化酶, 葡萄糖

Abstract：Instant and accurate detection of glucose level in blood and urine is of great significance to the early diagnosis and glucose monitoring of diabetes. Since gold nanocluster was found to have intrinsic phosphotransferaselike catalytic activity in 2004, various enzymelike activities were reported in nanomaterials (nanozymes), such as peroxidase, catalase, superoxide dismutase and oxidase. Compared with natural enzymes, nanozymes are of low cost, easy to be prepared, stored and transported, resistant to harsh conditions. Application of nanozymes in glucose detection will prospectively reduce the costs and improve the stability. The recent advances of various nanozymes including nanoparticles of metal, metal oxides, metal sulfides, carbon based materials and composite materials were introduced with emphasis on their advantages over natural enzymes, limits in the detection and linear working range in glucose detection. Challenges and prospects regarding the nanozyme research were also outlined in this review.

Key words： nanozyme peroxidase glucose oxidase glucose

基金资助:国家重点基础研究发展计划(973)项目（2015CB856302）

引用本文:

罗成¹李艳¹ 龙启文²龙建纲^3*. 纳米酶在葡萄糖分析检测中的应用研究进展[J]. 中国生物医学工程学报, 2016, 35(1): 105-113.
Luo Cheng¹Li Yan¹Long Qiwen²Long Jiangang^3*. Recent Advances of Nanozymes in Glucose Detection. journal1, 2016, 35(1): 105-113.

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http://cjbme.csbme.org/CN/ 10.3969/j.issn.0258-8021.2016. 01.013 或 http://cjbme.csbme.org/CN/Y2016/V35/I1/105

［1］American Diabetes A. Diagnosis and classification of diabetes mellitus ［J］. Diabetes Care, 2014, 37（Suppl 1）: S81-S90.
［2］Taylor R, Coulombe S, Otanicar T, et al. Small particles, big impacts: A review of the diverse applications of nanofluids ［J］. J Appl Phys, 2013, 113(1): 011301.
［3］Etheridge ML, Campbell SA, Erdman AG, et al. The big picture on nanomedicine: the state of investigational and approved nanomedicine products ［J］. NanomedNanotechnol, 2013, 9(1): 1-14.
［4］Manea F, Houillon FB, Pasquato L, et al. Nanozymes: goldnanoparticlebased transphosphorylation catalysts ［J］. Angew Chem Int Ed Engl, 2004, 43(45): 6165-6169.
［5］罗成, 李艳, 龙建纲. 纳米材料模拟酶的应用研究进展［J］. 中国科学化学, 2015, 45(10): 1026-1041.
［6］Xie Jianxin, Zhang Xiaodan, Wang Hui, et al. Analytical and environmental applications of nanoparticles as enzyme mimetics ［J］. TrAC, 2012, 39: 114-129.
［7］Wei Hui, Wang Erkang. Nanomaterials with enzymelike characteristics (nanozymes): nextgeneration artificial enzymes ［J］. Chem Soc Rev, 2013, 42(14): 6060-6093.
［8］He Weiwei, Zhou Yuting, Wamer WG, et al. Intrinsic catalytic activity of Au nanoparticles with respect to hydrogen peroxide decomposition and superoxide scavenging ［J］. Biomaterials, 2013, 34(3): 765-773.
［9］Luo Weijie, Zhu Changfeng, Su Shao, et al. Selfcatalyzed, selflimiting growth of glucose oxidasemimicking gold nanoparticles ［J］. ACS Nano, 2010, 4(12): 7451-7458.
［10］Jv Yun, Li Baoxin, Cao Rui. Positivelycharged gold nanoparticles as peroxidase mimic and their application in hydrogen peroxide and glucose detection ［J］. Chem Commun, 2010, 46(42): 8017-8019.
［11］Jiang Xin, Sun Cuiji, Guo Yi, et al. Peroxidaselike activity of apoferritin paired gold clusters for glucose detection ［J］. Biosens Bioelectron, 2015, 64: 165-170.
［12］Liu Jianbo, Hu Xiaona, Hou Shuai, et al. Au@Pt core/shell nanorods with peroxidase and ascorbate oxidaselike activities for improved detection of glucose ［J］. Sensor Actuat BChem, 2012, 166-167: 708-714.
［13］Li Yang, Ma Qiang, Liu Ziping, et al. A novel enzymemimic nanosensor based on quantum dotAu nanoparticle@silica mesoporous microsphere for the detection of glucose ［J］. Anal Chim Acta, 2014, 840: 68-74.
［14］Qu Konggang, Shi Peng, Ren Jinsong, et al. Nanocomposite incorporating V2O5 nanowires and gold nanoparticles for mimicking an enzyme cascade reaction and its application in the detection of biomolecules ［J］. Chem Eur J, 2014, 20(24): 7501-7506.
［15］Lin Youhui, Li Zhenhua, Chen Zhaowei, et al. Mesoporous silicaencapsulated gold nanoparticles as artificial enzymes for selfactivated cascade catalysis ［J］. Biomaterials, 2013, 34(11): 2600-2610.
［16］Lang NJ, Liu Biwu, Liu Juewen. Characterization of glucose oxidation by gold nanoparticles using nanoceria ［J］. J Colloid Interface Sci, 2014, 428: 78-83.
［17］Hu Lianzhe, Yuan Yali, Zhang Ling, et al. Copper nanoclusters as peroxidase mimetics and their applications to H2O2 and glucose detection ［J］. Anal Chim Acta, 2013, 762: 83-86.
［18］Jiang Huan, Chen Zhaohui, Cao Haiyan, et al. Peroxidaselike activity of chitosan stabilized silver nanoparticles for visual and colorimetric detection of glucose ［J］. Analyst, 2012, 137(23): 5560-5564.
［19］Chen Yujin, Cao Haiyan, Shi Wenbing, et al. FeCo bimetallic alloy nanoparticles as a highly active peroxidase mimetic and its application in biosensing ［J］. Chem Commun, 2013, 49(44): 5013-5015.
［20］Reddy LH, Arias JL, Nicolas J, et al. Magnetic nanoparticles: design and characterization, toxicity and biocompatibility, pharmaceutical and biomedical applications ［J］. Chem Rev, 2012, 112(11): 5818-5878.
［21］Gao Lizeng, Zhuang Jie, Nie Leng, et al. Intrinsic peroxidaselike activity of ferromagnetic nanoparticles ［J］. Nat Nanotechnol, 2007, 2(9): 577-583.
［22］Shi Wenbing, Zhang Xiaodan, He Shaohui, et al. CoFe2O4 magnetic nanoparticles as a peroxidase mimic mediated chemiluminescence for hydrogen peroxide and glucose ［J］. Chem Commun, 2011, 47(38): 10785-10787.
［23］Su Li, Feng Jie, Zhou Ximin, et al. Colorimetric detection of urine glucose based ZnFe2O4 magnetic nanoparticles ［J］. Anal Chem, 2012, 84(13): 5753-5758.
［24］Mitra K, Ghosh AB, Sarkar A, et al. Colorimetric estimation of human glucose level using gammaFe2O3 nanoparticles: an easily recoverable effective mimic peroxidase ［J］. Biochem Biophys Res Commun, 2014, 451(1): 30-35.
［25］Wei Hui, Wang Erkang. Fe3O4 magnetic nanoparticles as peroxidase mimetics and their applications in H2O2 and glucose detection ［J］. Anal Chem, 2008, 80(6): 2250-2254.
［26］Mu Jianshuai, Wang Yan, Zhao Min, et al. Intrinsic peroxidaselike activity and catalaselike activity of Co3O4 nanoparticles ［J］. Chem Commun, 2012, 48(19): 2540-2542.
［27］Liu Qingyun, Yang Yanting, Li Hui, et al. NiO nanoparticles modified with 5,10,15,20\|tetrakis(4\|carboxyl pheyl)
\|porphyrin: promising peroxidase mimetics for H2O2 and glucose detection ［J］. Biosens Bioelectron, 2015, 64: 147-153.
［28］Su Li, Qin Wenjie, Zhang Huige, et al. The peroxidase/catalaselike activities of MFe2O4 (M=Mg, Ni, Cu) MNPs and their application in colorimetric biosensing of glucose ［J］. Biosens Bioelectron, 2015, 63: 384-391.
［29］Liu Qingyun, Li Hui, Zhao Qingru, et al. Glucosesensitive colorimetric sensor based on peroxidase mimics activity of porphyrinFe3O4 nanocomposites ［J］. Mater Sci Eng C Mater Biol Appl, 2014, 41: 142-151.
［30］Liu Chenghao and Tseng Weilung. Oxidasefunctionalized Fe3O4 nanoparticles for fluorescence sensing of specific substrate ［J］. Anal Chim Acta, 2011, 703(1): 87-93.［31］Gao Yuan, Wang Guannan, Huang Hui, et al. Fluorometric method for the determination of hydrogen peroxide and glucose with Fe3O4 as catalyst ［J］. Talanta, 2011, 85(2): 1075-1080.
［32］Shi Yun, Su Ping, Wang Yingying, et al. Fe3O4 peroxidase mimetics as a general strategy for the fluorescent detection of H2O2-involved systems. Talanta, 2014, 130: 259-264.
［33］Kim MI, Shim J, Li Taihua, et al. Fabrication of nanoporous nanocomposites entrapping Fe3O4 magnetic nanoparticles and oxidases for colorimetric biosensing ［J］. Chem Eur J 2011, 17(38): 10700-10707.
［34］Liu Yan, Yuan Min, Qiao Longjiao, et al. An efficient colorimetric biosensor for glucose based on peroxidaselike protein Fe3O4 and glucose oxidase nanocomposites ［J］. Biosens Bioelectron, 2014, 52: 391-396.
［35］Xie Jianxin, Cao Haiyan, Jiang Huan, et al. Co3O4\|reduced graphene oxide nanocomposite as an effective peroxidase mimetic and its application in visual biosensing of glucose J］. Anal Chim Acta, 2013, 796: 92-100.
［36］Liu Qingyun, Zhu Renren, Du Hui, et al. Higher catalytic activity of porphyrin functionalized Co3O4 nanostructures for visual and colorimetric detection of H2O2 and gucose ［J］. Mater Sci Eng C Mater Biol Appl, 2014, 43: 321-329.
［37］Fan Yingwei and Huang Yuming. The effective peroxidaselike activity of chitosanfunctionalized CoFe2O4 nanoparticles for chemiluminescence sensing of hydrogen peroxide and glucose ［J］. Analyst, 2012, 137(5): 1225-1231.
［38］Zhao Minggang, Huang Jingyun, Zhou Yu, et al. Controlled synthesis of spinel ZnFe2O4 decorated ZnO heterostructures as peroxidase mimetics for enhanced colorimetri biosensing ［J］. Chem Commun, 2013, 49(69): 7656-7658.
［39］Chen Wei, Chen Juan, Feng Yebin, et al. Peroxidaselike activity of watersoluble cupric oxide nanoparticles and its analytical application for detection of hydrogen peroxide ad glucose ［J］. Analyst, 2012, 137(7): 1706-1712.
［40］Chen Wei, Hong Lei, Liu Ailin, et al. Enhanced chemiluminescence of the luminolhydrogen peroxide system by colloidal cupric oxide nanoparticles as peroxidase mimic J］. Talanta, 2012, 99: 643-648.
［41］Hu ailin, Liu Yinhuan, Deng Haohua, et al. Fluorescent hydrogen peroxide sensor based on cupric oxide nanoparticles and its application for glucose and Llactate detection ［J］. Biosens Bioelectron, 2014, 61: 374-378.
［42］Zhang Lingling, Han Lei, Hu Peng, et al. TiO2 nanotube arrays: intrinsic peroxidase mimetics ［J］. Chem Commun, 2013, 49(89): 10480-10482.
［43］Zhao Hui, Dong Yuming, Jiang Pingping, et al. Highly Dispersed CeO2 on TiO2 Nanotube: A Synergistic Nanocomposite with Superior PeroxidaseLike Activity ［J］. ACS Appl Mater Inter, 2015, 7(12): 6451-6461.
［44］Lin Tianran, Zhong Liangshuang, Guo Liangqia, et al. Seeing diabetes: visual detection of glucose based on the intrinsic peroxidaselike activity of MoS2 nanosheets ［J］. Nanoscale, 2014, 6(20): 11856-11862.
［45］Lin Tianran, Zhong Liangshuang, Song Zhiping, et al. Visual detection of blood glucose based on peroxidaselike activity of WS2 nanosheets ［J］. Biosens Bioelectron, 2014, 62: 302-307.
［46］Chen Qiao, Chen Jia, Gao Cunji, et al. Heminfunctionalized WS2 nanosheets as highly active peroxidase mimetics for labelfree colorimetric detection of H2O2 and glucose ［J］. Analyst, 2015, 140(8): 2857-2863.
［47］Dutta AK, Das S, Samanta S, et al. CuS nanoparticles as a mimic peroxidase for colorimetric estimation of human blood glucose level ［J］. Talanta, 2013, 107: 361-367.
［48］Liu Qingyun, Jia Qingyan, Zhu Renren, et al. 5,10,15,20\|Tetrakis(4\|carboxyl phenyl)porphyrinCdS nanocomposites with intrinsic peroxidaselike activity for glucos colorimetric detection ［J］. Mat Sci Eng CMater, 2014, 42: 177-184.
［49］He Weiwei, Jia Huimin, Li Xiaoxiao, et al. Understanding the formation of CuS concave superstructures with peroxidaselike activity ［J］. Nanoscale, 2012, 4(11): 3501-35
［50］Li Ruimin, Zhen Mingming, Guan Mirong, et al. A novel glucose colorimetric sensor based on intrinsic peroxidaselike activity of C60\|carboxyfullerenes ［J］. Biosens Bioelectron, 2013, 47: 502-507.
［51］Shi Wenbing, Wang Qinlong, Long Yijuan, et al. Carbon nanodots as peroxidase mimetics and their applications to glucose detection ［J］. Chem Commun, 2011, 47(23): 6695-6697.
［52］Wu Di, Deng Xiang, Huang Xiaomei, et al. Lowcost preparation of photoluminescent carbon nanodots and application as peroxidase mimetics in colorimetric detection of H2O2 and glucose ［J］. J Nanosci Nanotechno, 2013, 13〖STBZ〗(10): 6611-6616.
［53］Zheng Aixian, Cong Zhongxiao, Wang Jinru, et al. Highlyefficient peroxidaselike catalytic activity of graphene dots for biosensing ［J］. Biosens Bioelectron, 2013, 4: 519-524.
［54］Lin Tianran, Zhong Liangshuang, Wang Jing, et al. Graphitelike carbon nitrides as peroxidase mimetics and their applications to glucose detection ［J］. Biosens Bioelectron, 2014, 59: 89-93.
［55］Zhang Weimin, Ma Diao, Du Jianxiu. Prussian blue nanoparticles as peroxidase mimetics for sensitive colorimetric detection of hydrogen peroxide and glucose［J］. Talanta, 2014, 120: 362-367.
［56］Wang Ting, Fu Yingchun, Chai Liyuan, et al. Filling carbon nanotubes with Prussian blue nanoparticles of high peroxidaselike catalytic activity for colorimetric chemo and biosensing ［J］. Chem Eur J, 2014, 20(9): 2623-2630.
［57］Wan Lijuan, Liu Jinhuai, Huang Xingjiu. Novel magnetic nickel telluride nanowires decorated with thorns: synthesis and their intrinsic peroxidaselike activity for detection of glucose ［J］. Chem Commun, 2014, 50(88): 13589-13591.
［58］Wang Jingjing, Han Dongxue, Wang Xiaohong, et al. Polyoxometalates as peroxidase mimetics and their applications in H2O2 and glucose detection ［J］. Biosens Bioelectron, 2012, 36(1): 18-21.
［59］Chen Qiong, Liu Meiling, Zhao Jiangna, et al. Waterdispersible silicon dots as a peroxidase mimetic for the highlysensitive colorimetric detection of glucose ［J］. Chem Commun, 2014, 50(51): 6771-6774.
［60］Qiao Fengmin, Chen Lijian, Li Xueni, et al. Peroxidaselike activity of manganese selenide nanoparticles and its analytical application for visual detection of hydrogen peroxide and glucose ［J］. Sensor Actuat BChem, 2014, 193: 255-262.
［61］Wang Yixian, Zhang Xiao, Luo Zhimin, et al. Liquidphase growth of platinum nanoparticles on molybdenum trioxide nanosheets: an enhanced catalyst with intrinsic peroxidaselike catalytic activity ［J］. Nanoscale, 2014, 6(21): 12340-2344.
［62］Xing Zhicai, Tian Jingqi, Asiri AM, et al. Twodimensional hybrid mesoporous Fe2O3\|graphene nanostructures: a highly active and reusable peroxidase mimetic toward rapid, highly sensitive optical detection of glucose ［J］. Biosens Bioelectron, 2014, 52: 452-457.
［63］He Xiaolong, Tan Longfei, Chen Dong, et al. Fe3O4\|Au@mesoporous SiO2 microspheres: an ideal artificial enzymatic cascade system ［J］. Chem Commun, 2013, 49(41): 4643-4645.
［64］Dong Yalei, Zhang Huige, Rahman ZU, et al. Graphene oxide Fe3O4 magnetic nanocomposites with peroxidaselike activity for colorimetric detection of glucose ［J］. Nanoscale, 2012, 4(13): 3969-3976.
［65］Zheng Xuejing, Zhu Qian, Song Huiqing, et al. In situ synthesis of selfassembled threedimensional graphenemagnetic palladium nanohybrids with dualenzyme activity through onepot strategy and its application in glucose probe ［J］. ACS Appl Mater Inter, 2015, 7(6): 3480-3491.
［66］Chen Xi, Chen Jin, Wang Fubin, et al. Determination of glucose and uric acid with bienzyme colorimetry on microfluidic paperbased analysis devices ［J］. Biosens Bioelectron, 2012, 35(1): 363-368.
［67］Yu Faquan, Huang Yongzhuo, Cole AJ, et al. The artificial peroxidase activity of magnetic iron oxide nanoparticles and its application to glucose detection ［J］. Biomaterials, 2009, 30(27): 4716-4722.
［68］Nath S, Kaittanis C, Ramachandran V, et al. Synthesis, magnetic characterization and sensing applications of novel dextrancoated iron oxide nanorods ［J］. Chem Mater, 2009, 21(8): 1761-1767.
［69］Liu Shanhu, Lu Feng, Xing Ruimin, et al. Structural effects of Fe3O4 nanocrystals on peroxidaselike activity ［J］. Chem Eur J, 2011, 17(2): 620-625.
［70］赵晓华, 孟庆军, 毕春元, 等. 葡萄糖生物传感器研究进展［J］. 山东科学, 2009, 22(2): 34-38.
［71］杨秀云, 梁凤, 张巍, 等. 葡萄糖生物传感器检测方法的研究进展［J］. 应用化学, 2012, 29(12): 1364-1370.