低强度脉冲超声波联合引导骨再生术对Beagle犬牙周骨开窗缺损修复效应的研究

doi:10.3969/j.issn.0258-8021.2012.06.014

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摘要本研究探讨了低强度脉冲超声波(LIPUS)联合引导骨再生术(GBR)，对Beagle犬牙周骨开窗缺损的修复效应。构建5只Beagle犬尖牙颊侧根中1/3处5 mm×5 mm矩形牙周骨开窗缺损模型，将每只Beagle犬4颗尖牙随机分配到4个治疗组：LIPUS（90 mW/cm²,20 min/d）组、LIPUS（90 mW/cm²,20 min/d）+ GBR组、GBR组、空白对照组，每组共计5颗实验牙。4周后处死Beagle犬，通过对实验区进行HE、Masson染色、Micro-CT检测以及对新生牙槽骨面积（NBA）及新生牙槽骨占初始缺损面积百分比（NBA%）的测量，比较各组牙周骨开窗缺损的组织学修复差异。Micro-CT观察显示，各组新生牙槽骨面积由大到小依次为LIPUS+GBR组、GBR组、LIPUS组、空白对照组；Masson染色显示，相比GBR组和空白对照组，LIPUS组和LIPUS+GBR组新生牙槽骨胶原成熟度较高；组织学测量各组NBA及NBA%，LIPUS组为(0.39±0.06) mm²，7.74%±1.09%；LIPUS+GBR组为(0.52±0.13) mm²，10.3%±2.22%；GBR组为(0.41±0.11) mm²，7.44%±2.20%；空白对照组为(0.24±0.04) mm²，4.64%±0.99%，各组间牙槽骨修复效果差异有统计学意义（P＜0.05），新生牙槽骨量由大到小依次为LIPUS+GBR组、GBR组、LIPUS组、空白对照组。LIPUS辅助GBR治疗能够促进牙周骨开窗缺损的早期修复。

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	高翔¹宋锦璘^1*邓锋¹赵纯亮²王智彪²

关键词 ：低强度脉冲超声波, 引导骨组织再生, Beagle犬, 牙周骨开窗缺损, 修复

Abstract：The aim of this work is to investigate the effect of combined application of lowintensity pulsed ultrasound (LIPUS) and guided bone regeneration (GBR) on canine periodontal fenestration defect in Beagle dogs. In the experiments, five Beagle dogs were used for establishing 5 mm×5 mm periodontal fenestration defect. Four canines in each dog were randomly divided into LIPUS（90 mW/cm², 20 min/d）group, LIPUS（90 mW/cm², 20 min/d）+ GBR group, GBR group and Control group. Each group had 5 teeth. In order to compare the tissue repair among the groups, the specimens of periodontal fenestration were obtained for histological analysis (HE and Masson staining), Micro-CT test and histometric analysis (the measurement of new bone area, NBA and its percentage in initial defect area, NBA%) after 4 weeks. Micro-CT test results showed that the area of new alveolar bone from large to small was LIPUS+GBR group, GBR group, LIPUS group, and Control group. Histological analysis showed that the Masson staining of new bone in LIPUS and LIPUS+GBR group was mainly red while GBR and control group was mainly blue. It suggested the new bone collagen in LIPUS group and LIPUS+GBR group was more mature than in GBR group and control group. Histometric analysis showed that NBA and NBA% were 0.39 mm² and 7.74% in respectively LIPUS group; 0.52 mm² and 10.30% respectively in LIPUS+GBR group; 0.41 mm² and 7.44% respectively in GBR group; 0.24 mm² and 4.64% respectively in Control group. Differences between groups were significant (P＜0.05). It suggested the amount of new alveolar bone from large to small was LIPUS+GBR group, GBR group, LIPUS group, and Control group. LIPUS combined with guided bone regeneration (GBR) can promote early repair of periodontal fenestration defect.

Key words： low-intensity pulsed ultrasound guided bone regeneration beagle dog periodontal fenestration defect repair

基金资助:国家自然科学基金(30870754)；重庆市卫生局科研项目（20102228）；重庆市科委自然科学基金（CSTC2010BB5355）

引用本文:

高翔¹宋锦璘^1*邓锋¹赵纯亮²王智彪². 低强度脉冲超声波联合引导骨再生术对Beagle犬牙周骨开窗缺损修复效应的研究[J]. 中国生物医学工程学报, 2012, 31(6): 895-901.
GAO Xiang¹SONG Jin Lin^1*DENG Feng¹ZHAO Chun Liang²WANG Zhi Biao^2. Effect of LowIntensity Pulsed Ultrasound Combined with Guided Bone Regeneration on Canine Periodontal Fenestration Defect in Beagle Dogs. journal1, 2012, 31(6): 895-901.

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http://cjbme.csbme.org/CN/10.3969/j.issn.0258-8021.2012.06.014 或 http://cjbme.csbme.org/CN/Y2012/V31/I6/895

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