三维多孔电磁复合支架构建与理化表征

doi:10.3969/j.issn.0258-8021.2019.04.9

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Abstract Objective: To mimic the tissue structure and physiological function of extracellular matrix (ECM) to fabricate electromagnetic nanofibrous scaffolds that are favorable for cell adhesion, proliferation and differentiation.Nanofibrous films were fabricated by coaxial electrospinning with gelatin as shell layer and polylactic acid (PLA) as core layer; meanwhile, magnetic nanoparticles (MNP) were added into the gelatin and PLA. The obtained nanofibrous films were pulverized and blended with carbon fiber (CF), followed by freeze-drying and crosslinking treatment to obtain three-dimensional (3D) porous scaffolds. The morphology and structure of the scaffolds were observed by scanning electron microscope (SEM) and transmission electron microscope (TEM). The hysteresis loop was measured by vibrating sample magnetometer, the conductivity was measured using four point probe, and compression tests were carried out by universal material testing machine. The density and water absorption were also measured. CCK-8 and Western Blot were used to analyze the viability and function of the cells on the scaffolds. The scaffolds showed porous honeycomb microstructure, the pores were perforated with eachother. When CF content was 0, 1, 3 and 5 mg/mL, the density of scaffolds was 73.07, 72.56, 65.88 and 63.34 mg/cm³, the water absorption was 1164.60%, 1186.48%, 1284.835% and 1323.66%, the conductivity was 0, 0.0088, 0.2467 and 2.6625 s/m, and the saturated magnetization strength was 3.68, 3.15, 2.45 and 2.90 emu/g, respectively. The scaffolds up-regulated the expression of Cx43 and RhoA, as well as supported the growth of cardiomyocytes. The 3D porous electromagnetic composite scaffolds had both superparamagnetism and conductivity, the mechanical property was significantly improved by CF. The scaffolds supported growth and promoted the maturation of cardiomyocytes.

Key words： electromagnetic composite scaffolds extracellular matrix carbon fiber cardiomyocyte

Received: 17 April 2018

PACS:

R318

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	Articles by authors
	Xu Xuegai
	Wu Fengxin
	Gao Aijun
	Meng Jie
	Wen Tao
	Liu Jian
	Xu Lianghua
	Xu Haiyan

Cite this article:

Xu Xuegai,Wu Fengxin,Gao Aijun, et al. Fabrication and Characterization of Three-Dimensional Porous Electromagnetic Composite Scaffolds[J]. Chinese Journal of Biomedical Engineering, 2019, 38(4): 447-454.

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http://cjbme.csbme.org/EN/10.3969/j.issn.0258-8021.2019.04.9 OR http://cjbme.csbme.org/EN/Y2019/V38/I4/447

[1] Langer R, Vacanti JP. Tissue engineering [J]. Science, 1993, 260(5110): 920-926.
[2] Langer R, Vacanti JP. Advances in tissue engineering [J]. Journal of Pediatric Surgery, 2016, 51(1): 8-12.
[3] Xu Tao, Miszuk JM, Zhao Yong, et al. Electrospun polycaprolactone 3D nanofibrous scaffold with interconnected and hierarchically structured pores for bone tissue engineering [J]. Advanced Healthcare Materials, 2015, 4(15): 2238-2246.
[4] Xu He, Li Haiyan, Ke Qingfei, et al. An anisotropically and heterogeneously aligned patterned electrospun scaffold with tailored mechanical property and improved bioactivity for vascular tissue engineering [J]. Acs Appl Mater Interfaces, 2015, 7(16): 8706-8718.
[5] Kroschwitz JI. Encyclopedia of Polymer Science and Technology [M]. Third ed. Hoboken: John Wiley & Sons, 2005: 261-291.
[6] Kishan AP, Cosgriff-Hernandez EM. Recent advancements in electrospinning design for tissue engineering applications: A review [J]. Journal of Biomedical Materials Research Part A, 2017, 105(10): 2892-2905.
[7] Chen Weiming, Ma Jun, Zhu Lei, et al. Superelastic, superabsorbent and 3D nanofiber-assembled scaffold for tissue engineering [J]. Colloids and Surfaces B: Biointerfaces, 2016: 165-172.
[8] Pok S, Vitale F, Eichmann SL, et al. Biocompatible carbon nanotube-chitosan scaffold matching the electrical conductivity of the heart [J]. ACS Nano, 2014, 8(10): 9822-9832.
[9] Oliveira JM, Pina S, Reis LR, et al. Osteochondral tissue engineering [M]// Ortega Z, Alemán ME, Donate R. Nanofibers and Microfibers for Osteochondral Tissue Engineering. Berlin: Springer Verlag, 2018: 97-123.
[10] Wu Fengxin, Gao Aijun, Liu Jian, et al. High modulus conductive hydrogels enhance in vitro vaturation and contractile function of primary cardiomyocytes for uses in drug screening [J]. Advanced Healthcare Materials, 2018, 7(24):1800-1990.
[11] Yun HM, Ahn SJ, Park KR, et al. Magnetic nanocomposite scaffolds combined with static magnetic field in the stimulation of osteoblastic differentiation and bone formation [J]. Biomaterials, 2016, 85: 88-98.
[12] Lin HY, Lin YJ. In vitro effects of low frequency electromagnetic fields on osteoblast proliferation and maturation in an inflammatory environment [J]. Bioelectromagnetics, 2011, 32(7): 552-560.
[13] Hao Suisui, Meng Jie, Zhang Yu, et al. Macrophage phenotypic mechanomodulation of enhancing bone regeneration by superparamagnetic scaffold upon magnetization [J]. Biomaterials, 2017, 140(140): 16-25.
[14] Meng Jie, Xiao Bo, Zhang Yu, et al. Super-paramagnetic responsive nanofibrous scaffolds under static magnetic field enhance osteogenesis for bone repair in vivo [J]. Scientific Reports, 2013, 3(1): 2655-2655.
[15] Sisson K, Zhang C, Farachcarson MC, et al. Evaluation of cross-linking methods for electrospun gelatin on cell growth and viability [J]. Biomacromolecules, 2009, 10(7): 1675-1680.
[16] Knobel M, Nunes WC, Socolovsky LM, et al. Superparamagnetism and other magnetic features in granular materials: a review on ideal and real systems [J]. Journal of Nanoscience and Nanotechnology, 2010, 40(43): 2836-2857.
[17] Sun Yongkang, Ma Ming, Zhang Yu, et al. Synthesis of nanometer-size maghemite particles from magnetite [J]. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 2004, 245(13): 15-19.
[18] Potse M, Dube B, Vinet A. Cardiac anisotropy in boundary-element models for the electrocardiogram [J]. Med Biol Eng Comput, 2009, 47 (7): 719-729.
[19] Beauchamp P, Desplantez T, Mccain ML, et al. Electrical coupling and propagation in engineered ventricular myocardium with heterogeneous expression of connexin43 [J]. Circulation Research, 2012, 110(11): 1445-1453.
[20] Schulz R, Gorge PM, Gorbe A, et al. Connexin 43 is an emerging therapeutic target in ischemia/reperfusion injury, cardioprotection and neuroprotection [J]. Pharmacology & Therapeutics, 2015, 153: 90-106.
[21] Mitra SK, Hanson DA, Schlaepfer DD. Focal adhesion kinase: in command and control of cell motility [J]. Nature Reviews Molecular Cell Biology, 2005, 6(1): 56-68.
[22] Santoro M, Shah SR, Walker JL, et al. Poly(lactic acid) nanofibrous scaffolds for tissue engineering [J]. Advanced Drug Delivery Reviews, 2016, 107: 206-212.
[23] Li Keguo, Wang Yun, Miao Zhechuan, et al. Chitosan/gelatin composite microcarrier for hepatocyte culture [J]. Biotechnology Letters, 2004, 26(11): 879-883.
[24] Lin HY, Lin YJ. In vitro effects of low frequency electromagnetic fields on osteoblast proliferation and maturation in an inflammatory environment [J]. Bioelectromagnetics, 2011, 32(7):552-560.
[25] Liang Haiwei, Guan Qingfang, Chen Lifeng, et al. Macroscopic-scale template synthesis of robust carbonaceous nanofiber hydrogels and aerogels and their applications [J]. Angewandte Chemie, 2012, 51(21): 5101-5105.
[26] You J, Rafat M, Ye GJ, et al. Nanoengineering the heart: conductive scaffolds enhance connexin 43 expression [J]. Nano Letters, 2011, 11(9): 3643-3648.