聚乙二醇-b-聚己内酯/聚己内酯单分散电喷微球的制备与亲水性研究

doi:10.3969/j.issn.0258-8021.2019.01.010

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Abstract This study is aimed to prepare monodispersed and hydrophilic microspheres with Poly(ethylene glycol)-b-poly (ε-caprolactone) / Polycaprolactone (PEG-b-PCL/PCL) by electrospraying. PEG-b-PCL and PCL was dissolved in chloroform to prepare a mixture solution, which was magnetically stirred for 3 hours. The influence of the content of PEG-b-PCL, flow rateand the voltage of the electrospraying on the morphology, size, and size distribution of microspheres were investigated, The influence of PEG-b-PCL on hydrophilicity of microspheres and the dispersion of the microspheres in water was investigated. Monodispersed spherical microspheres with average particle sizes ranging from 5 to 6 μm and the coefficient of variation ranging from 15%～21% could be fabricated when the content of PEG-b-PCL was 10%～20%, flow rate was 1mL/h, and voltage was 10 kV. Microspheres along with fibers could be fabricated when the content of PEG-b-PCL was 30%. The contact angle of the microspheres decreased from 126.2°±4.8° to 29.9°±4.9° when the content of PEG-b-PCL was increased from 0 to 20%, and the differences showed statistical significance (P<0.05), indicating that changing the content of PEG-b-PCL could improve the hydrophilicity of the microspheres. In the meantime, microspheres with 10%～20% of PEG-b-PCL could form homogeneous dispersions in water. Conclusion: Monodispersed microspheres with improved hydrophilicity could be fabricated with PEG-b-PCL/PCL, which would be a foundation for further research on hydrophilic drug-loaded microspheres.

Key words： microspheres poly(ethylene glycol)-b-poly (ε-caprolactone) polycaprolactone monodis-persed hydrophilicity

Received: 13 January 2018

PACS:

R318

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	Articles by authors
	Yang Xue
	Shao Huaying
	Zhang Qiongyue
	Wu Xiaohong

Cite this article:

Yang Xue,Shao Huaying,Zhang Qiongyue, et al. Preparation and Hydrophilic Characterization of Monodispersed PEG-b-PCL/ PCL Microspheres by Electrospraying[J]. Chinese Journal of Biomedical Engineering, 2019, 38(1): 77-83.

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http://cjbme.csbme.org/EN/10.3969/j.issn.0258-8021.2019.01.010 OR http://cjbme.csbme.org/EN/Y2019/V38/I1/77

[1] Diniz IMA,Chen C,Ansari S, et al. Gingival mesenchymal stem cell (gmsc) delivery system based on rgd-coupled alginate hydrogel with antimicrobial properties: a novel treatment modality for peri-implantitis[J]. Journal of Prosthodontics, 2016, 25(2): 105-115.
[2] Renvert S,Lessem J,Dahlén G, et al. Topical minocycline microspheres versus topical chlorhexidine gel as an adjunct to mechanical debridement of incipient peri‐implant infections: a randomized clinical trial[J]. Journal of Clinical Periodontology, 2006, 33(5): 362-369.
[3] Nguyen DN,Clasen C,Van DMG. Pharmaceutical applications of electrospraying[J]. Journal of Pharmaceutical Sciences, 2016, 105(9): 2601-2620.
[4] Wu F, Wei J, Liu C, et al. Fabrication and properties of porous scaffold of zein/pcl biocomposite for bone tissue engineering[J]. Composites Part B, 2012, 43(5): 2192-2197.
[5] Jin G,Prabhakaran MP,Kai D, et al. Tissue engineered plant extracts as nanofibrous wound dressing[J]. Biomaterials, 2013, 34(3): 724-734.
[6] Schuckert KH,Jopp S,Teoh SH. Mandibular defect reconstruction using three-dimensional polycaprolactone scaffold in combination with platelet-rich plasma and recombinant human bone morphogenetic protein-2: denovo synthesis of bone in a single case[J]. Tissue Eng Part A, 2009, 15(3): 493-499.
[7] Woodruff MA,Hutmacher DW. The return of a forgotten polymer—polycaprolactone in the 21st century[J]. Progress in Polymer Science, 2010, 35(10): 1217-1256.
[8] Edlund U,Albertsson A. Morphology engineering of a novel poly(l-lactide)/poly(1,5-dioxepan-2-one) microsphere system for controlled drug delivery[J]. Journal of Polymer Science Part A Polymer Chemistry, 2015, 38(5): 786-796.
[9] Ji Y,Wang L,Watts DC, et al. Controlled-release naringin nanoscaffold for osteoporotic bone healing[J]. Dental Materials Official Publication of the Academy of Dental Materials, 2014, 30(11): 1263-1273.
[10] Gaucher G,Dufresne MH,Sant VP, et al. Block copolymer micelles: preparation, characterization and application in drug delivery.[J]. Journal of Controlled Release, 2005, 109(1): 169-188.
[11] 纪艳,王璐,游涛,等. 柚皮苷纳米纤维膜载药量影响成骨细胞的增殖和分化[J].中国组织工程研究,2013,17(25):4577-4584.
[12] 胡倩. 聚乙二醇—聚己内酯(mPEG-PCL)的血液相容性研究[D]. 广州: 暨南大学,2016.
[13] Bock N,Woodruff MA,Hutmacher DW, et al. Electrospraying, a reproducible method for production of polymeric microspheres for biomedical applications[J]. Polymers, 2011, 3(1): 131-149.
[14] Park CH,Lee J. Electrosprayed polymer particles: effect of the solvent properties[J]. Journal of Applied Polymer Science, 2010, 114(1): 430-437.
[15] Almería, Deng W, Fahmy TM, et al. Controlling the morphology of electrospray-generated plga microparticles for drug delivery.[J]. Colloid Interface Sci, 2010, 343(1): 125-133.
[16] Torres-giner S,Martinez-abad A,Ocio MJ, et al. Stabilization of a nutraceutical omega-3 fatty acid by encapsulation in ultrathin electrosprayed zein prolamine[J]. Journal of Food Science, 2010, 75(6): N69-N79.
[17] Enayati M,Ahmad Z,Stride E, et al. One-step electrohydrodynamic production of drug-loaded micro- and nanoparticles[J]. Journal of the Royal Society Interface, 2010, 7(45): 667-675.
[18] Hu JF,Li SF,Nair GR, et al. Predicting chitosan particle size produced by electrohydrodynamic atomization[J]. Chemical Engineering Science, 2012, 82(82): 159-165.
[19] Jafari-Nodoushan M,Barzin J,Mobedi H. Size and morphology controlling of plga microparticles produced by electro hydrodynamic atomization[J]. Polymers for Advanced Technologies, 2015, 26(5): 502-513.
[20] Nam J,Huang Y,Agarwal S, et al. Improved cellular infiltration in electrospun fiber via engineered porosity[J]. Tissue Engineering Part A, 2007, 13(9): 2249-2257.
[21] Desai KG. Chitosan nanoparticles prepared by ionotropic gelation: an overview of recent advances[J]. Critical Reviews in Therapeutic Drug Carrier Systems, 2016, 33(2): 107-158.