小鼠背部皮肤的在体阻抗谱实验研究

doi:10.3969/j.issn.0258-8021. 2017. 02.007

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Abstract Different from other tissues, it is difficult to study the impedance of stratum corneum regarding skin as a whole subject, due to its huge impedance. The aim of this work is to non-invasively examine the characteristics of the impedance of mice’s back skin including stratum corneum in vivo. Using Agilent 4294A impedance analyzer, impedance spectrums of the back skin of 15 C57BL/6 mice under anesthesia were measured, and surface electrodes and acupuncture needles were used to record data as the whole skin and viable skin respectively. Along with the increase of frequency, the amplitude of impedance of the whole skin declined at the rate of -20 dB/dec within all the frequency span from 40 Hz to 10⁷ Hz; whereas that of the viable skin decreased at -10 dB/dec in the range of 40 Hz to10³ Hz, and at -3 dB/dec in the range of 10³ Hz to 10⁷ Hz. The phase of the whole skin decreased and then increased in a V-shape curve, and that of viable skin varied as a Π-shape curve. At low frequencies, such as 100 Hz, the whole skin impedance can be regarded as only consisting of the impedance of stratum corneum, and the viable skin part can be omitted. At medium frequencies, such as 10⁵ Hz, the viable skin contributes over 10% to the whole skin impedance. However, at high frequencies, such as 10⁷ Hz, the viable skin contributed over 80% to the whole skin impedance. The impedance of the whole skin and viable skin showed significant difference below 10³ Hz (P<0.05) and no significant difference over 10⁴ Hz. The results showed that this novel method that comparing the impedance recorded by surface electrodes and acupuncture needles was effective in the analysis of the impedance of stratum corneum within the whole skin impedance.

Key words： mice skin stratum corneum impedance in vivo

Received: 19 July 2016

PACS:

R318

About author:: (Senior member, Chinese Society of Biomedical Engineering)

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	Li Ying
	Song Bing
	Liu Zhipeng
	Yin Tao

Cite this article:

Li Ying,Song Bing,Liu Zhipeng, et al. Experimental Study on in vivo Impedance Spectrums of Mice's Back Skin[J]. Chinese Journal of Biomedical Engineering, 2017, 36(2): 172-179.

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http://cjbme.csbme.org/EN/10.3969/j.issn.0258-8021. 2017. 02.007 OR http://cjbme.csbme.org/EN/Y2017/V36/I2/172

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