Development of Chemiluminescence Immune Detection System Based on Photomultiplier Tube
1 Shanghai University for Science and Technology, Mechanical Engineering department, Shanghai 200093, China
2 Tongji University,Shanghai 200092, China
3 State Engineering Lab of Immunoassay,Shanghai 200093, China
Abstract In this work a full automatic chemiluminescence immune analyzer was designed and fabricated base on photomultiplier tube (PMT). Experiments were conducted in which the prolactin (PRL) was taken as a detection object, the Horse Radish Peroxidase (HRP) served as Markey enzyme, and the Pierce Luminol (excitation substrate) was employed as a light resource. The PMT data obtained by the chemiluminescence immune detection system was processed, calibrated through the number of light emitting detection of five different consistency of PRL.The relationship between the consistency of PRL and the lightemitting was obtained. Samples in positive range of consistency (sample A) and negative range of consistency (sample B) and samples for LUMO and Liaison were measured. The results of sample A obtained from the present system, LUMO and Liaison were 51.724, 46.198, 43.792 ng/mL respectively, the data of sample B were 11.702, 15.133, 8.069 ng/mL respectively. It showed that the automatic chemiluminescence immune system can measure the negative or positive of samples and meet the application requirements of the immunoassay through the comparison with other chemiluminescence analyzers.
SUN Fu Jia1,2,3*CHEN Shuo Shuo1 CAI Jin Da1, et al. Development of Chemiluminescence Immune Detection System Based on Photomultiplier Tube[J]. journal1, 2012, 31(6): 859-865.
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