基于光电倍增管的化学发光免疫检测系统的研制

doi:10.3969/j.issn.0258-8021.2012.06.009

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Abstract In this work a full automatic chemiluminescence immune analyzer was designed and fabricated base on photomultiplier tube (PMT). Experiments were conducted in which the prolactin (PRL) was taken as a detection object, the Horse Radish Peroxidase (HRP) served as Markey enzyme, and the Pierce Luminol (excitation substrate) was employed as a light resource. The PMT data obtained by the chemiluminescence immune detection system was processed, calibrated through the number of light emitting detection of five different consistency of PRL.The relationship between the consistency of PRL and the lightemitting was obtained. Samples in positive range of consistency (sample A) and negative range of consistency (sample B) and samples for LUMO and Liaison were measured. The results of sample A obtained from the present system, LUMO and Liaison were 51.724, 46.198, 43.792 ng/mL respectively, the data of sample B were 11.702, 15.133, 8.069 ng/mL respectively. It showed that the automatic chemiluminescence immune system can measure the negative or positive of samples and meet the application requirements of the immunoassay through the comparison with other chemiluminescence analyzers.

Key words： chemiluminescence immunoassay analyzer (CLIA) photomultiplier tube (PMT) signal acquisition system

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	Articles by authors
	SUN Fu Jia¹
	2
	3*CHEN Shuo Shuo¹CAI Jin Da¹
	3YOU Qian Lin¹

Cite this article:

SUN Fu Jia^1,2,3*CHEN Shuo Shuo¹CAI Jin Da^{1, et al. Development of Chemiluminescence Immune Detection System Based on Photomultiplier Tube[J]. journal1, 2012, 31(6): 859-865.}

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http://cjbme.csbme.org/EN/10.3969/j.issn.0258-8021.2012.06.009 OR http://cjbme.csbme.org/EN/Y2012/V31/I6/859

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