|
|
|
| An Experimental Study on the Behavior of Streaming Potentials in Bone Microchannels |
| School of Mechanical Engineering Tianjin University, Tianjin 300072, China |
|
|
|
|
Abstract Mechanical loading is an important regulatory factor for bone mass and structure. Bone remodeling and formation are closely associated to metabolism. It is considered that electromechanical property of bone is a link between bone remodeling and mechanical loading, therefore, the streaming potential is one important direction in the electromechanical property of bone. The objective of this study is to confirm the difference of the produced streaming potential from buffer solution flowing through Haversian canal and bone canalicules by experiments. The streaming potential of eight bone specimens under five different loading rates was measured using selfdesigned testing system. The lateral surface of bone specimen was sealed by silicone rubber to simulate the flowing situation of buffer solution flowing through Haversian canal; while unsealed condition was used to simulate the situation of buffer solution flowing through Haversian canal and bone canalicules of osteon whose specimen surface was split at the same time. Result showed that corresponding to loading rates of 26, 36, 60, 180 and 360 kPa/s, when the lateral surface was sealed, the stable values of streaming potential respectively were (0.29±0.09），（0.24±0.06），（0.21±0.05），（0.19±0.05）and（0.16±0.04）mV. Under unsealed condition, the streaming potential was (069±008）,（0.61±0.09）,（0.57±0.07）,（0.51±0.05）and（0.46±0.05）mV, which was apparently higher than that under sealed condition (P < 0.05). Besides, the difference between the potentials reflects that the produced streaming potential when the buffer solution was flowing through bone canalicules was higher than that when the buffer solution was flowing through Haversian canal. Based on the microstructure of bone and in consideration of bone cells are mainly distributed near the bone canalicules, the experimental results provide the basis for study of streaming potential and bone remodeling.
|
|
|
|
|
|
［1］Wu Xiaogang, Chen Weiyi, Gao Zhipeng, et al. The effects of Haversian fluid pressure and harmonic axial loading on the poroelastic behaviors of a single osteon ［J］. Science China: Physics, Mechanics and Astronomy, 2012, 55(19): 1646-1656.
［2］Bassett CA, Becker RO. Generation of electric potentials by bone in response to mechanical stress ［J］. Science, 1962, 137: l063-1064.
［3］Anderson JC, Eriksson C. Electrical properties of wet collagen ［J］. Nature, 1968, 218(5137): 166-168.
［4］Riddle RC, Donahue HJ. From streaming potentials to shear stress: 25 years of bone cell mechanotransduction ［J］. Journal of Orthopedic Research, 2009, 27(2): 143-149.
［5］Pienkowski D, Pollack SR. The origin of stressgenerated potentials in fluidsaturated bone ［J］. Journal of Orthopedic Research, 1983, 1(1): 30-41.
［6］Zhu Junjie, Davidson Christian, Xuan Xiangchun. Flow ratemodified streaming effects in heterogeneous microchannels ［J］. Microfluidics and Nanofluidics, 2008, 5(6): 733740.
［7］AFT Mak, Zhang Jiangdan. Numerical simulation of streaming potentials due to deformationinduced hierarchical flows in cortical bone ［J］, Biomech Eng, 2001, 123(1): 66-70.［8］Lemaire T, Nali S, Rémond A. Study of the influence of fibrous pericellular matrix in the cortical interstitial fluid movement with hydroelectrochemical effects ［J］. Journal of Biomechanical Engineering, 2008, 130(1): 1-11.
［9］Lemaire T, Nali S, Rémond A. Multiscale analysis of the coupled effects governing the movement of interstitial fluid in cortical bone ［J］. Biomechanics and Modeling in Mechanobiology, 2006, 5(1): 39-52.
［10］Lemaire T, CapiezLernout E, Kaiser J, et al. A multiscale theoretical investigation of electric measurements in living bone piezoelectricity and electrokinetics ［J］. Bulletin of Mathematical Biology, 2011, 73(11): 2649-2677.
［11］Qin Yunxuan, Lin Wan, Rubin C. The pathway of bone fluid flow as defined by in vivo intramedullary pressure and streaming potential measurements ［J］. Annals of Biomedical Engineering, 2002, 30(5): 693-702.
［12］Hong J, Ko SO, Khang G, et al. Intraosseous pressure and strain generated potential of cylindrical bone samples in the drained uniaxial condition for various loading rates ［J］. Journal of Materials Science: Materials in Medicine, 2008, 19(7): 2589-2594.
［13］Xu Lianyun, Hou Zhende, Wang Hong. Investigation of pressure loading rates on streaming potentials in bone ［J］. Science China Technological Sciences, 2011, 54(6): 1376-1381.
［14］Quenneville E, Binette JS. Fabrication and characterization of nonplanar microelectrode array circuits for use in arthroscopic diagnosis of cartilage diseases ［J］, IEEE Transactions on Biomedical Eng, 2004, 51(12): 2164-2173.
［15］Garon M, Legare A, Guardo R. Streaming potentials maps are spatially resolved indicators of amplitude, frequency and ionic strength dependant responses of articular cartilage to load ［J］, Journal of Biomechanics, 2002, 35(2): 207-216.
［16］Hastings GW, Mahmud FA. Electrical effects in bone ［J］. Journal of Biomedical Engineering, 1988, 10(6): 515-521.
［17］Lu Fuzhi, How Tuck Y, Daniel Y Kwok. An improved method for determining zeta potential and pore conductivity of porous materials ［J］. Journal of Colloid and Interface Science, 2006, 299(2): 972-976.
［18］徐莲云，侯振德，钟声，等. 加载速率对骨的流动电位的影响 ［J］. 实验力学, 2009, 24(4): 320-326. |
|
|
|
