乳腺癌瘤床可降解标记物在大鼠体内的安全性和有效性

doi:10.3969/j.issn.0258-8021.2025.02.008

Abstract
Figure/Table
References
Related Citation (15)

Download: PDF (4129 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract The aim of this investigation is to evaluate the safety and efficacy of breast cancer tumor-bed biodegradable markers in rats. In experiments, a total of 100 rats were divided into surgical group, titanium clip group and degradable labeling group (n=30), and the other 10 rats were set up for the whole degradable observation group. The biodegradable, buckle-shaped marker with round holes and grooves were made of PLA/β-TCP material, and the markers and titanium clips were implanted into the rat mammary tissue. Hematoxylin and eosin staining (HE) and immunohistochemistry (IHC) were used to analyze the inflammatory responses of rat perimammary tissues after marker implantation. Using CT and MR imaging, the degradable markers and titanium clips were observed for the presence of artifacts, and the ability of the degradable markers to achieve effective visualization over the observation period (46 weeks) was evaluated. Safety results showed that the acute immune responses in the tissues around the degradable markers were higher than that of the titanium clips and returned to normal after 12 weeks. The gap between the expression of IL-6 in the degradable labeling group and the titanium clips group was reduced from 12% to 2.8%. The gap between the expression of IL-10 in the degradable labeling group and the titanium clips group was reduced from 14.8% to 8.1%. Statistical analysis showed that the difference in the rate of positive cells for inflammatory factors was significant in the first 4 weeks. The validity results showed that the boundaries of the degradable markers were clear without obvious artifacts on CT and MR images, and the CT values tended to decrease gradually with the increase of time. The results showed that the inflammatory reaction induced by the degradable marker in rats basically disappeared at week 12, and the imaging effect on CT and MR was better than that of the titanium clip marker. In the future, this new biodegradable marker could potentially be an alternative to titanium clips.

Key words： degradable marker breast tumor tumor bed

Received: 27 March 2024

PACS:

R318

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	Xie Xuejie
	Xie Xin
	Luo Yingen
	Kong Xiangyi
	Wang Ningyu
	Dai Jianrong

Cite this article:

Xie Xuejie,Xie Xin,Luo Yingen, et al. Evaluation of the Safety and Efficacy of Breast Cancer Tumor-Bed Degradable Markers in Rats[J]. Chinese Journal of Biomedical Engineering, 2025, 44(2): 203-210.

URL:

http://cjbme.csbme.org/EN/10.3969/j.issn.0258-8021.2025.02.008 OR http://cjbme.csbme.org/EN/Y2025/V44/I2/203

[1] Jonczyk MM, Jean J, Graham R, et al. Surgical trends in breast cancer: a rise in novel operative treatment options over a 12 year analysis[J]. Breast Cancer Res Treat, 2019,173(2):267-274.
[2] Kirby AN, Jena R, Harris EJ, et al. Tumour bed delineation for partial breast/breast boost radiotherapy: what is the optimal number of implanted markers? [J]. Radiother Oncol, 2013,106(2):231-235.
[3] Demircioglu O, Aribal E, Uluer M, et al. Surgical clips in breast-conserving surgery: do they represent the tumour bed accurately? [J]. Curr Med Imaging Rev, 2019,15(6):573-577.
[4] 罗娇娇,彭海燕,罗焕丽等.保乳术后放疗中钛夹动度与乳腺大小及钛夹位置的相关性[J].中国医学物理学杂志,2022,39(5):529-534.
[5] Polgár C, Fodor J, Major T, et al. Radiotherapy confined to the tumor bed following breast conserving surgery current status, controversies, and future projects[J]. Strahlenther Onkol, 2002,178(11):597-606.
[6] Shaikh T, Chen Ting, Khan A, et al. Improvement in interobserver accuracy in delineation of the lumpectomy cavity using fiducial markers[J]. Int J Radiat Oncol Biol Phys, 2010,78(4):1127-1134.
[7] Handsfield LL, Yue NingJ, Zhou Jinghao, et al. Determination of optimal fiducial marker across image-guided radiation therapy (IGRT) modalities: visibility and artifact analysis of gold, carbon, and polymer fiducial markers[J]. J Appl Clin Med Phys, 2012,13(5):3976.
[8] Ciernik IF, Greiss AM. Visualization of the tumor cavity after lumpectomy of breast cancer for postoperative radiotherapy[J]. Clin Transl Radiat Oncol, 2018,14:47-50.
[9] Struik GM, Hoekstra N, Klem TM, et al. Pignol Pilot study on the use of a radiopaque hydrogel during breast conserving surgery with full-thickness closure for target definition of boost or partial breast irradiation [J]. Brachytherapy, 2018,17 (4): S52-S53.
[10] Foster B, Sindhu K, Hepel J, et al. Three-Dimensional bioabsorbable tissue marker placement is associated with decreased tumor bed volume among patients receiving radiation therapy for breast cancer[J]. Pract Radiat Oncol, 2019,9(2): e134-e141.
[11] Piper M, Peled AW, Sbitany H. Oncoplastic breast surgery: current strategies[J]. Gland Surg, 2015,4(2):154-163.
[12] Ciernik IF, Greiss AM. Visualization of the tumor cavity after lumpectomy of breast cancer for postoperative radiotherapy[J]. Clin Transl Radiat Oncol. 2018,14:47-50.
[13] Srour MK, Chung A. Utilization of BioZorb implantable device in breast-conserving surgery[J]. Breast J. 2020,26(5):960-965.
[14] 厉虹.聚乳酸/β-TCP复合材料制备与性能研究[D].南京:东南大学,2017.
[15] Uemura T, Kojima H. Bone formation in vivo induced by Cbfa1-carrying adenoviral vectors released from a biodegradable porous β-tricalcium phosphate (β-TCP) material[J]. Sci Technol Adv Mater. 2011,12(3):034405.
[16] Li Chunlin, Shwu JC, Shyh MK, et al. Preparation and evaluation of β-TCP/polylactide microspheres as osteogenesis materials[J]. Science and Technology of Advanced Materials, 2008,108(5):3210-3217.
[17] 谢欣,张凯旋,陈辛元,等.可用于保乳手术瘤床标记物的生物可降解材料的筛选研究[J].中国生物医学工程学报,2022,41(5):637-640.
[18] Benonisson H, Altıntaş I, Sluijter M, et al. CD3-bispecific antibody therapy turns solid tumors into inflammatory sites but does not install protective memory[J]. Mol Cancer Ther, 2019,18(2):312-322.
[19] Xie Ping, Zhang Minghua, He Shan, et al. The covalent modifier Nedd8 is critical for the activation of Smurf1 ubiquitin ligase in tumorigenesis[J]. Nat Commun,2014,5:3733.
[20] Li L, Chen L, Lin F, et al. Study of the expression of inflammatory factors IL-4, IL-6, IL-10, and IL-17 in liver failure complicated by coagulation dysfunction and sepsis[J]. J Inflamm Res,2021,15(14):1447-1453.
[21] Ciernik IF, Greiss AM. Visualization of the tumor cavity after lumpectomy of breast cancer for postoperative radiotherapy[J]. Clin Transl Radiat Oncol,2018,14:47-50.