异氟烷麻醉对小鼠自发肌电及TUS/TMAS诱发肌电的影响

doi:10.3969/j.issn.0258-8021.2024.01.002

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Abstract Transcranial ultrasound stimulation (TUS) and transcranial magneto-acoustic stimulation (TMAS) are effective in modulating motor cortex. Previous studies have mostly been conducted under anesthesia confined to the difficulty of tethering conscious animals, and the analysis of the attenuating modulatory effects of anesthesia has primarily focused on the central nervous system. In this study, spontaneous electromyography (EMG) and TUS/TMAS-induced EMG were recorded during isoflurane anesthesia in 24 mice, the effects of anaesthetize on spontaneous and induced EMG were analyzed quantitatively in terms of firing rate, latency, duration and amplitude. Experimental results showed that the frequency of spontaneous EMG decreased by 50% per cycle approximately and the duration decreased, as the concentration of isoflurane increased from 0.4% to 0.75%, indicating an inhibitory state. The induced-EMG success rates of TUS/TMAS both decreased by 50% and 70% respectively, with an average increase of 0.1 s in latency and a decrease of 0.3 s and 0.5 s in duration respectively, suggesting that the modulatory effect of TUS/TMAS on the motor cortex attenuated as the depth of anesthesia increased. According to the observed correlation between the firing rate and duration of spontaneous and induced EMG, it is rational to infer that the suppression of spontaneous EMG in mice under anesthesia was one of the factors contributing to the attenuated modulatory effect.

Key words： transcranial ultrasound stimulation transcranial magneto-acoustic stimulation electromyography (EMG) anesthesia

Received: 17 May 2023

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R318

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	Articles by authors
	Wang Ruru
	Zhou Xiaoqing
	Zhao Yuhui
	Liu Xu
	Liu Zhipeng
	Wang Xin
	Yin Tao

Cite this article:

Wang Ruru,Zhou Xiaoqing,Zhao Yuhui, et al. Effects of Isoflurane Anesthesia on Spontaneous and TUS/TMAS Induced Electromyography in Mice[J]. Chinese Journal of Biomedical Engineering, 2024, 43(1): 10-17.

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http://cjbme.csbme.org/EN/10.3969/j.issn.0258-8021.2024.01.002 OR http://cjbme.csbme.org/EN/Y2024/V43/I1/10

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