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| Research Progress of 3D Printing Organ-on-a-Chip |
| Luo Zhiming1,2,3,4, Deng Guohao1, Wang Zhubing1, Zhang Yujie2,3,4, Zhang Yang1*, Wang Huanan2,3,4* |
1(School of Biomedical Engineering, Shenzhen University Health Science Center, Shenzhen 518055, Guangdong, China)
2(State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024, Liaoning, China)
3(Frontiers Science Center for Smart Materials Oriented Chemical Engineering, Dalian University of Technology, Dalian 116024, Liaoning, China)
4(School of Bioengineering, Dalian University of Technology, Dalian 116024, Liaoning, China) |
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Abstract Organ-on-a-chip is a biomimetic microphysiological system that recapitulates certain functions of tissues and organs by seeding live cells in delicately designed microfluidic chips. Organ-on-a-chip has a wide application in disease simulation, toxicity detection, new drug development and screening, personalized medical treatment, etc. The application of 3D printing organ-on-a-chip realizes the simplification and low cost of the chip manufacture and meet the delicate requirements of complex heterogeneous three-dimensional microenvironments. This review updated the recent progress in 3D printing organ-on-a-chip from the aspects of printing methods, printing inks and their biomedical applications. We reviewed the printing methods and ink materials for the construction of structural and functional units of organ chips and discussed the potential to develop more efficient one-step manufacture of organ chip. At last, we summarized the recent progress of using 3D-printed organ chip in bionic heart, liver, lung, kidney, nerve, tumor models, and outlook the future trend of organ-on-a-chip techniques.
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Received: 08 July 2021
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Corresponding Authors:
* E-mail: yang.zhang@szu.edu.cn;huananwang@dlut.edu.cn
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[1] Huh D, Matthews BD, Mammoto A, et al. Reconstituting organ-level lung functions on a chip [J]. Science, 2010, 328(5986): 1662-1668.
[2] Caplin JD, Granados NG, James MR, et al. Microfluidic organ-on-a-chip technology for advancement of drug development and toxicology [J]. Advanced Healthcare Materials, 2015, 4(10): 1426-1450.
[3] Seder I, Kim DM, Hwang SH, et al. Microfluidic chip with movable layers for the manipulation of biochemicals [J]. Lab on a Chip, 2018, 18(13): 1867-1874.
[4] 刘妍, 杨清振, 陈小明, 等. 3D打印技术制备器官芯片的研究现状 [J]. 中国生物医学工程学报, 2020, 39(1): 97-108.
[5] Gale B, Jafek A, Lambert C, et al. A review of current methods in microfluidic device fabrication and future commercialization prospects [J]. Inventions, 2018, 3(3): 60-85.
[6] Wu Jing, Gu Min. Microfluidic sensing: state of the art fabrication and detection techniques [J]. Journal of Biomedical Optics, 2011, 16(8): 080901-080912.
[7] Zhang Bin, Gao Lei, Ma Liang, et al. 3D bioprinting: a novel avenue for manufacturing tissues and organs [J]. Engineering, 2019, 5(4): 777-794.
[8] Zhang YS, Arneri A, Bersini S, et al. Bioprinting 3D microfibrous scaffolds for engineering endothelialized myocardium and heart-on-a-chip [J]. Biomaterials, 2016, 110: 45-59.
[9] Mehrotra S, de Melo BA, Hirano M, et al. Nonmulberry silk based ink for fabricating mechanically robust cardiac patches and endothelialized myocardium-on-a-chip application [J]. Advanced Functional Materials, 2020, 30(12): 1907436-1907451.
[10] Bhise NS, Manoharan V, Massa S, et al. A liver-on-a-chip platform with bioprinted hepatic spheroids [J]. Biofabrication, 2016, 8(1): 014101-014110.
[11] Lee H, Cho DW. One-step fabrication of an organ-on-a-chip with spatial heterogeneity using a 3d bioprinting technology [J]. Lab on a Chip, 2016, 16(14): 2618-2625.
[12] Zhang Jie, Chen Fengming, He Ziyi, et al. A novel approach for precisely controlled multiple cell patterning in microfluidic chips by inkjet printing and the detection of drug metabolism and diffusion [J]. Analyst, 2016, 141(10): 2940-2947.
[13] Ma Xuanyi, Qu Xin, Zhu Wei, et al. Deterministically patterned biomimetic human ipsc-derived hepatic model via rapid 3D bioprinting [J]. Proceedings of the National Academy of Sciences of the United States of America, 2016, 113(8): 2206-2211.
[14] Horvath L, Umehara Y, Jud C, et al. Engineering an in vitro air-blood barrier by 3d bioprinting [J]. Scientific Reports, 2015, 5(1): 1-8.
[15] Park JY, Ryu H, Lee B, et al. Development of a functional airway-on-a-chip by 3D cell printing [J]. Biofabrication, 2019, 11(1): 015002-015010.
[16] Homan KA, Kolesky DB, Skylar-Scott MA, et al. Bioprinting of 3D convoluted renal proximal tubules on perfusable chips [J]. Scientific Reports, 2016, 6: 1-13.
[17] Gong Hua, Bickham BP, Woolley AT, et al. Custom 3d printer and resin for 18 mum x 20 mum microfluidic flow channels [J]. Lab on a Chip, 2017, 17(17): 2899-2909.
[18] Santana HS, Palma MSA, Lopes MGM, et al. Microfluidic devices and 3D printing for synthesis and screening of drugs and tissue engineering [J]. Industrial & Engineering Chemistry Research, 2020, 59(9): 3794-3810.
[19] Ridky TW, Chow JM, Wong DJ, et al. Invasive three-dimensional organotypic neoplasia from multiple normal human epithelia [J]. Nature Medicine, 2010, 16(12): 1450-1455.
[20] Loessner D, Stok KS, Lutolf MP, et al. Bioengineered 3D platform to explore cell-ecm interactions and drug resistance of epithelial ovarian cancer cells [J]. Biomaterials, 2010, 31(32): 8494-8506.
[21] Li Chunli, Tian Tao, Nan Kejun, et al. Survival advantages of multicellular spheroids vs. Monolayers of hepg2 cells in vitro [J]. Oncology Reports, 2008, 20(6): 1465-1471.
[22] Friedl P, Wolf K. Plasticity of cell migration: a multiscale tuning model [J]. Journal of Cell Biology, 2010, 188(1): 11-19.
[23] Godoy P, Hewitt NJ, Albrecht U, et al. Recent advances in 2D and 3D in vitro systems using primary hepatocytes, alternative hepatocyte sources and non-parenchymal liver cells and their use in investigating mechanisms of hepatotoxicity, cell signaling and adme [J]. Archives of Toxicology, 2013, 87(8): 1315-1530.
[24] Gonzalez G, Chiappone A, Dietliker K, et al. Fabrication and functionalization of 3D printed polydimethylsiloxane-based microfluidic devices obtained through digital light processing [J]. Advanced Materials Technologies, 2020, 5(9): 2000374-2000380.
[25] Knowlton S, Yu CH, Ersoy F, et al. 3d-printed microfluidic chips with patterned, cell-laden hydrogel constructs [J]. Biofabrication, 2016, 8(2): 02019-02027.
[26] 周丽宏, 陈自强, 黄国友, 等. 细胞打印技术及应用 [J]. 中国生物工程杂志, 2010, 30(12): 95-104.
[27] Cui Xiaofeng, Boland T, D′Lima DD, et al. Thermal inkjet printing in tissue engineering and regenerative medicine [J]. Recent Patents on Drug Delivery & Formulation, 2012, 6(2): 149-155.
[28] 吴懋亮, 诸文俊, 李涤尘, 等. 光固化成型中的变形分析 [J]. 西安交通大学学报, 1999, 33(9): 90-97.
[29] Melchels FPW, Feijen J, Grijpma DW. A review on stereolithography and its applications in biomedical engineering [J]. Biomaterials, 2010, 31(24): 6121-6130.
[30] Amin R, Knowlton S, Hart A, et al. 3D-printed microfluidic devices [J]. Biofabrication, 2016, 8(2): 022001-0220018.
[31] Bedell ML, Navara AM, Du Yingying, et al. Polymeric systems for bioprinting [J]. Chem Rev, 2020, 120(19): 10744-10792.
[32] Urrios A, Parra-Cabrera C, Bhattacharjee N, et al. 3D-printing of transparent bio-microfluidic devices in peg-da [J]. Lab on a Chip, 2016, 16(12): 2287-2294.
[33] Morris VB, Nimbalkar S, Younesi M, et al. Mechanical properties, cytocompatibility and manufacturability of chitosan: Pegda hybrid-gel scaffolds by stereolithography [J]. Annals of Biomedical Engineering, 2017, 45(1): 286-296.
[34] Kotz F, Arnold K, Bauer W, et al. Three-dimensional printing of transparent fused silica glass [J]. Nature, 2017, 544(7650): 337-339.
[35] Bhattacharjee N, Parra-Cabrera C, Kim YT, et al. Desktop-stereolithography 3D-printing of a poly(dimethylsiloxane)-based material with sylgard-184 properties [J]. Adv Mater, 2018, 30(22): 1800001-1800008.
[36] Munoz-Pinto DJ, Jimenez-Vergara AC, Gharat TP, et al. Characterization of sequential collagen-poly (ethylene glycol) diacrylate interpenetrating networks and initial assessment of their potential for vascular tissue engineering [J]. Biomaterials, 2015, 40: 32-42.
[37] McManus MC, Boland ED, Koo HP, et al. Mechanical properties of electrospun fibrinogen structures [J]. Acta Biomaterialia, 2006, 2(1): 19-28.
[38] Lee HY, Hwang CH, Kim HE, et al. Enhancement of bio-stability and mechanical properties of hyaluronic acid hydrogels by tannic acid treatment [J]. Carbohydrate Polymers, 2018, 186: 290-298.
[39] Drury JL, Dennis RG, Mooney DJ. The tensile properties of alginate hydrogels [J]. Biomaterials, 2004, 25(16): 3187-3199.
[40] Fu Jing, Yang Fuchao, Guo Zhiguang. The chitosan hydrogels: from structure to function [J]. New Journal of Chemistry, 2018, 42(21): 17162-17180.
[41] Pathak A, Kumar S. Independent regulation of tumor cell migration by matrix stiffness and confinement [J]. Proceedings of the National Academy of Sciences, 2012, 109(26): 10334-10339.
[42] Homan KA, Kolesky DB, Skylar-Scott MA, et al. Bioprinting of 3D convoluted renal proximal tubules on perfusable chips [J]. Scientific Reports, 2016, 6(1): 1-13.
[43] Zhang Xue, Morits M, Jonkergouw C, et al. Three-dimensional printed cell culture model based on spherical colloidal lignin particles and cellulose nanofibril-alginate hydrogel [J]. Biomacromolecules, 2020, 21(5): 1875-1885.
[44] Toepke MW, Beebe DJ. Pdms absorption of small molecules and consequences in microfluidic applications [J]. Lab on a Chip, 2006, 6(12): 1484-1486.
[45] Gong Hua, Bickham BP, Woolley AT, et al. Custom 3D printer and resin for 18 μm x 20 μm microfluidic flow channels [J]. Lab Chip, 2017, 17(17): 2899-2909.
[46] Sun Wei, Starly B, Daly AC, et al. The bioprinting roadmap [J]. Biofabrication, 2020, 12(2): 022002-022036.
[47] Murphy SV, Atala A. 3D bioprinting of tissues and organs [J]. Nature Biotechnology, 2014, 32(8): 773-785.
[48] Meng Hongxu, Chowdhury TT, Gavara N. The mechanical interplay between differentiating mesenchymal stem cells and gelatin-based substrates measured by atomic force microscopy [J]. Frontiers in Cell and Developmental Biology, 2021, 9: 1639-1649.
[49] Griffith LG. Polymeric biomaterials [J]. Acta Mater, 2000, 48(1): 263-277.
[50] Hubbell JA. Bioactive biomaterials [J]. Current Opinion in Biotechnology, 1999, 10(2): 123-129.
[51] Lee H, Cho DW. One-step fabrication of an organ-on-a-chip with spatial heterogeneity using a 3D bioprinting technology [J]. Lab on a Chip, 2016, 16(14): 2618-2625.
[52] Yu Fang, Zhuo Shuangmu, Qu Yinghua, et al. On chip two-photon metabolic imaging for drug toxicity testing [J]. Biomicrofluidics, 2017, 11(3): 034108-034120.
[53] No DY, Lee K-H, Lee J, et al. 3D liver models on a microplatform: Well-defined culture, engineering of liver tissue and liver-on-a-chip [J]. Lab on a Chip, 2015, 15(19): 3822-3837.
[54] Tong Wenhao, Fang Yu, Yan Jie, et al. Constrained spheroids for prolonged hepatocyte culture [J]. Biomaterials, 2016, 80: 106-120.
[55] Jenkinson SE, Chung GW, van Loon E, et al. The limitations of renal epithelial cell line hk-2 as a model of drug transporter expression and function in the proximal tubule [J]. Pflugers Archiv-European Journal of Physiology, 2012, 464(6): 601-611.
[56] Johnson BN, Lancaster KZ, Hogue IB, et al. 3d printed nervous system on a chip [J]. Lab on a Chip, 2016, 16(8): 1393-1400.
[57] Bowser DA, Moore MJ. Biofabrication of neural microphysiological systems using magnetic spheroid bioprinting [J]. Biofabrication, 2019, 12(1): 015002-015014.
[58] King SM, Presnell SC, Shepherd BR. Development of 3D bioprinted human breast cancer for in vitro screening of therapeutics targeted against cancer progression [J]. Molecular Biology of the Cell, 2013, 74(19): 2014-2034.
[59] Zhao Yu, Yao Rui, Ouyang Liliang, et al. Three-dimensional printing of hela cells for cervical tumor model in vitro [J]. Biofabrication, 2014, 6(3): 035001-035010.
[60] Skardal A, Murphy SV, Devarasetty M, et al. Multi-tissue interactions in an integrated three-tissue organ-on-a-chip platform [J]. Sci Rep, 2017, 7(1): 8837-8853.
[61] Bhattacharjee N, Urrios A, Kang S, et al. The upcoming 3D-printing revolution in microfluidics[J]. Lab on a Chip, 2016, 16(10): 1720-1742.
[62] Calvert P. Inkjet printing for materials and devices [J]. Chemistry of Materials, 2001, 13(10): 3299-3305.
[63] Erkal JL, Selimovic A, Gross BC, et al. 3D printed microfluidic devices with integrated versatile and reusable electrodes [J]. Lab on a Chip, 2014, 14(12): 2023-2032.
[64] Begolo S, Zhukov DV, Selck DA, et al. The pumping lid: investigating multi-material 3D printing for equipment-free, programmable generation of positive and negative pressures for microfluidic applications [J]. Lab on a Chip, 2014, 14(24): 4616-4628.
[65] Paydar OH, Paredes CN, Hwang Y, et al. Characterization of 3D-printed microfluidic chip interconnects with integrated o-rings [J]. Sensors and Actuators a-Physical, 2014, 205: 199-203.
[66] Bonyár A, Sántha H, Ring B, et al. 3D Rapid Prototyping Technology (RPT) as a powerful tool in microfluidic development[J]. Procedia Engineering, 2010, 5: 291-294.
[67] Comina G, Suska A, Filippini D. Pdms lab-on-a-chip fabrication using 3D printed templates [J]. Lab on a Chip, 2014, 14(2): 424-430.
[68] Nie Jing, Fu Jianzhong, He Yong. Hydrogels: The next generation body materials for microfluidic chips? [J]. Small, 2020, 16(46): 2003797-2003819.
[69] Hinton TJ, Hudson A, Pusch K, et al. 3D printing pdms elastomer in a hydrophilic support bath via freeform reversible embedding [J]. ACS Biomaterials Science & Engineering, 2016, 2(10): 1781-1786.
[70] Bhattacharjee N, Parra-Cabrera C, Kim YT, et al. Desktop-stereolithography 3D-printing of a poly (dimethylsiloxane)-based material with sylgard-184 properties [J]. Advanced Materials, 2018, 30(22): 1800001-1800008. |
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