Abstract The development of injectable dynamic hydrogels has been one hot issue in recent years, and the preparation of catalyst-free fast-crosslinked injectable dynamic hydrogels is still one big challenge. In this paper, reversible dynamic Schiff base bonds and electrostatic interactions, formed by amine groups on methylacrylated chitosan (CHMA) molecules with aldehyde groups and carboxyl groups on Aldehyde hyaluronic acid (ALHA) molecules respectively, were utilized to prepare a novel catalyst-free hydrogel. The gelation rate, shear thinning behavior and self-healing characteristics of the hydrogel were characterized by dynamic rheological analyzer, the cytocompatibility was evaluated by the cell three-dimensional culture experiment, and the wound healing rate was evaluated through the acute full-thickness skin wound repair experiment. Results showed that the hydrogel quickly formed in 5 seconds the aqueous solution of CHMA and ALHA were mixed. In addition, the hydrogel was an injectable one with shear thinning and rapid self-healing. The complex viscosity gradually decreased from 0.4 kPa to 8 Pa, when the scanning frequency increased from 10-1 s-1 to 102 s-1. Meanwhile, the values order of its storage modulus and loss modulus switched quickly between 1% and 1000% of the strain, yet the modulus had no significant decrease. The hydrogel also exhibited excellent cytocompatibility and fast wound healing rate, evidenced by the high cell survival rate (>95%) in the 3D culture of cells, and a reduction in the wound healing time of 5-7 days in the hydrogel group compared to the blank control based on a model of acute full-thickness skin defect in male ICR mice. The injectable chitosan-hyaluronate hydrogel showed broad application perspectives in the fields of regenerative medicine.
Wu Yidong,Hong Dan,Hao Wenjuan, et al. Ultra-Rapid Fabrication of Dynamic-Crosslinked Injectable Chitosan-Hyaluronate Hydrogel for Wound Healing[J]. Chinese Journal of Biomedical Engineering, 2021, 40(5): 590-596.
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