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| HeLa Cells Uptake of Silica Coated Gold Nanorods and Intracellular Localization Based on Optical Imaging |
| Sang Xiang1,2, Wang Kexin1,2, Xiao Shuanghuang3,4, Yang Hongqin1,2, Peng Yiru3,4, Chen Jianling1,2* |
1(College of Photonic and Electronic Engineering,Fujian Normal University,Fuzhou 350007,China)
2(Key Lab of Optoelectronic Science and Technology for Medicine of Ministry of Education,Fujian Provincial Key Lab for Photonics Technology,Fuzhou 350007,China)
3(College of Chemistry and Materials Science,Fujian Normal University,Fuzhou 350007,China)
4(Fujian Provincial Key Laboratory of Polymer Materials,Fuzhou 350007,China) |
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Abstract Gold nanorods exhibit outstanding optical properties and high photothermal conversion efficiency,making them widely used in biomedical imaging and treatment. When gold nanorods are used in clinical treatments,their interaction with cells is a key issue. In this paper,the toxicity of silica coated gold nanorods to HeLa cells was studied. When HeLa cells were cultured with silica coated gold nanorods for different incubation time (4 h,8 h,12 h,24 h),the cellular uptake and intracellular distribution were observed by the two photon microscopy. We found out that the toxicity of silica coated gold nanorods to HeLa cells was incubation time and concentration dependent,and the serum in culture media could reduce the cytotoxicity. When silica coated gold nanorods were incubated with HeLa cells for 12 h,the viability of cells in serum-containing media was close to 100%,and in the serum free medium,the viability of cells was about 85% when the concentration of silica coated gold nanorods was1 250 μg/mL. After incubated for 24 h with 50 μg/mL of silica coated gold nanorods,the viability of cells in the medium with or without serum was 99.0% and 85.1%,respectively. However,the viability of cells cultured with or without serum decreased to 58.3% and 31.2%,respectively when the concentration of silica coated gold nanorods was 1 250 μg/mL. The serum in the culture medium prevented HeLa cells from taking up the silica coated gold nanorods,and the uptake was time-dependent. The internalized silica coated gold nanorods mainly accumulated in the lysosomes,did not enter the mitochondria. The study could provide a reference for the imaging and treatment of cervical cancer with gold nanorods in the future.
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Received: 02 August 2019
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