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| Study on the Mechanism of Tannic Acid Inducing Bovine Jugular Vein Cross-Linking |
| Zheng Xiaobing1, Zhou Jianye1*, Wang De1, Dai Zehui2, Guo Baohua2 |
1(State Key Laboratory of Cardiovascular Disease, Fu Wai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100037, China)
2(Key Laboratory of Advanced Materials, Ministry of Education, Department of Chemical Engineering, Tsinghua University, Beijing 100037, China) |
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Abstract The aim of this study was to investigate chemical reactions of tannic acid (TA) with bovine jugular vein (BJV). Glutaraldehyde-fixed BJVs were subsequently treated with TA or four substitutional compounds, which contained four characteristic chemical groups of tannic acid. The treated BJVs were implanted subcutaneously into 20 SD rats for 21 and 60 days. The calcium content of the explanted tissues was assessed using atomic absorption spectrometry, and the degree of cross-linking was measured by Masson and EVG staining. In vitro, they were characterized with thermal stability, resistance to enzymatic hydrolysis, mechanical properties. The Fourier transform infrared spectroscopy (FTIS) was employed to inspect the changes of absorption curve. Furthermore, a gradient concentration of urea (0.1, 0.3, 0.5 and 1 M) was used to verify if hydrogen bond was formed. Tannic acid treated BJV, with polyphenolic hydroxyl groups in the compound, showed the best performance among the all BJVs. Which suggested effective cross-linking was formed in the tissue, and the calcium content at 21 d and 60 d was 2.25 mg/g and 8.26 mg/g respectively (P< 0.05). FTIS results indicated that hydrogen bond was formed in TA group and it was confirmed by urea test. The urea test showed that the effect of cross-linking between tissue decreased (P< 0.05) with the increase of urea concentration, and the calcium content of TA group was 8.10 mg/g at 60 d, which was much lower than 16.83 mg/g of the 0.1 M urea group and 50.76 mg/g of the 0.5 M urea group(P < 0.001). Our results demonstrated that the hydrogen bond was formed between polyphenolic hydroxyl groups and proteins in TA treated BJVs. It may provide a clue to improve the stability of the reaction system.
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Received: 20 December 2018
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Corresponding Authors:
E-mail:zhoujy@263.net
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[1] Shahanavaz S, Qureshi AM, Levi DS, et al. Transcatheter pulmonary valve replacement with the melody valve in small diameter expandable right ventricular outflow tract conduits [J]. JACC: Cardiovascular Interventions, 2018, 11(6): 554-564.
[2] Emani SM, Piekarski BL, Zurakowski D, et al. Concept of an expandable cardiac valve for surgical implantation in infants and children [J]. J Thorac Cardiovasc Surg, 2016, 152(6): 1514-1523.
[3] Gonzalez Rocafort A, Aroca A, Abelleira C, et al. Stented bovine jugular vein graft (melody valve) in mitral position. Could be an alternative for mechanical valve replacement in the pediatric population? [J]. Rev Esp Cardiol (engl ed), 2017, 70(8): 675-677.
[4] Abdullah I, Ramirez FB, McElhinney DB, et al. Modification of a stented bovine jugular vein conduit (melody valve) for surgical mitral valve replacement [J]. Ann Thorac Surg, 2012, 94(4): e97-e98.
[5] Jayakrishnan SRJ. Glutaraldehyde as a fixative in bioprostheses and drug delivery matrices. [J]. Biomaterids, 1996, 17:471-484.
[6] Isenburg JC, Simionescu DT, Vyavahare NR. Tannic acid treatment enhances biostability and reduces calcification of glutaraldehyde fixed aortic wall [J]. Biomaterials, 2005, 26(11): 1237-1245.
[7] 王德, 周建业, 胡盛寿,等. 丹宁酸改性处理对戊二醛固定牛颈静脉管道理化特性的影响 [J]. 中国医学科学院学报, 2009, 31(1): 65-67.
[8] 孙嘉康, 周建业, 唐跃,等. 丹宁酸交联后续处理对牛颈静脉血管的影响 [J]. 中国比较医学杂志, 2016, 26(5): 86-92.
[9] Isenburg JC, Simionescu DT, Vyavahare NR. Elastin stabilization in cardiovascular implants: improved resistance to enzymatic degradation by treatment with tannic acid [J]. Biomaterials, 2004, 25(16): 3293-3302.
[10] Isenburg JC, Karamchandani NV, Simionescu DT, et al. Structural requirements for stabilization of vascular elastin by polyphenolic tannins [J]. Biomaterials, 2006, 27(19): 3645-3651.
[11] Guo Gaoyang, Jin Linhe, Jin Wanyu, et al. Radical polymerization-crosslinking method for improving extracellular matrix stability in bioprosthetic heart valves with reduced potential for calcification and inflammatory response [J]. Acta Biomater, 2018, 82:44-55.
[12] Moore MA, Chen WM, Phillips RE, et al. Shrinkage temperature versus protein extraction as a measure of stabilization of photooxidized tissue [J]. J Biomed Mater Res, 1996, 32(2): 209-214.
[13] Leong J, Munnelly A, Liberio B, et al. Neomycin and carbodiimide crosslinking as an alternative to glutaraldehyde for enhanced durability of bioprosthetic heart valves [J]. J Biomater Appl, 2013, 27(8): 948-960.
[14] Tripi D R, Vyavahare N R. Neomycin and pentagalloyl glucose enhanced cross-linking for elastin and glycosaminoglycans preservation in bioprosthetic heart valves [J]. J Biomater Appl, 2014, 28(5): 757-766.
[15] 黄磊,匡锋,孔德淼,等. 两种交联技术处理的兔去细胞颈动脉性能比较 [J]. 中南大学学报(医学版), 2018, 43(4): 441-446.
[16] Chang Y, Hsu CK, Wei HJ, et al. Cell-free xenogenic vascular grafts fixed with glutaraldehyde or genipin: in vitro and in vivo studies [J]. J Biotechnol, 2005, 120(2): 207-219.
[17] Jin Wanyu, Guo Gaoyang, Chen Liang, et al. Elastin stabilization through polyphenol and ferric chloride combined treatment for the enhancement of bioprosthetic heart valve anticalcification [J]. Artif Organs, 2018, 42(11): 1062-1069.
[18] Simionescu DT. Prevention of calcification in bioprosthetic heart valves: challenges and perspectives [J]. Expert Opin Biol Ther, 2004, 4(12): 1971-1985.
[19] Yu SY. Cross-linking of elastin in human atherosclerotic aortas. I. A preliminary study [J]. Laboratory Investigation, 1971, 25(2): 121-125.
[20] Hinds MT, Courtman DW, Goodell T, et al. Biocompatibility of a xenogenic elastin-based biomaterial in a murine implantation model: the role of aluminum chloride pretreatment [J]. J Biomed Mater Res A, 2004, 69(1): 55-64.
[21] Luck G, Liao H, Murray NJ, et al. Polyphenols, astringency and proline-rich proteins [J]. Phytochemistry, 1994, 37(2): 357-371.
[22] Ejima H, Richardson JJ, Liang K, et al. One-step assembly of coordination complexes for versatile film and particle engineering [J]. Science, 2013, 341(6142): 154-157.
[23] Yang Liwei, Han Lulu, Ren Jun, et al. Coating process and stability of metal-polyphenol film [J]. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 2015, 484:197-205.
[24] Yang Liwei, Han Lulu, Liu Qi, et al. Galloyl groups-regulated fibrinogen conformation: Understanding antiplatelet adhesion on tannic acid coating [J]. Acta Biomater, 2017, 64:187-199. |
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