Abstract：To meet the requirement of intelligent morphology classification of compound colonies, a convolution neural network for colony classification was proposed in this work. All connected domains in the culture dish were obtained by level set evolution. Limit corrosion was used to determine the connected domains that seed points were greater than one. Obtaining the convex closures of the connected domains, the concave points were detected. By connecting the corresponding concave points, the connected domains with adhesion were segmented. Herein 600 single colony samples were normalized, and the data were expanded to 30,000 cases by rotating and flipping and superimposing random noise with signal-to-noise ratio not exceeding 5%. Samples of 70% were used as the training set for the network. The network was cross-verified by 10 folds. Samples of 30% were used as the test set. The weighted average accuracy of the four colonies reached 87.50%, of which the classification accuracy of spotted smooth colonies was 86.40%, that of circle like wavy colonies was 87.21%, that of oval colonies was 88.11%, and that of irregular other colonies was 87.25%. Compute unified device architecture (CUDA) was adopted to accelerate the algorithm. The running time was optimized to 1/10 of the original time. In terms of running speed and convenience, the algorithm far exceeded the traditional methods. The statistical results showed that the proposed method could effectively complete the task of intelligent classification and identification of compound colonies. This method showed good expansibility and self-learning function, which had reference value for intelligent analysis of biochemical samples based on images.
余辉, 杜培培, 刘祥, 刘政, 朱险峰, 曹玉珍. 基于卷积神经网络的复合菌落智能分类识别[J]. 中国生物医学工程学报, 2020, 39(1): 26-32.
Yu Hui, Du Peipei, Liu Xiang, Liu Zheng, Zhu Xianfeng, Cao Yuzhen. Intelligent Classification and Identification of Composite Colonies Based on Convolutional Neural Network. Chinese Journal of Biomedical Engineering, 2020, 39(1): 26-32.
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