Fabrication of Puerarin@FeS2/Ti3C2 Nanoplatform for Postoperative Bone Tumor Therapy
Qing You 1, Li Suiyan1*, Weng Jie2#*
1(College of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China) 2(College of Medicine (Institute of Biomedical Engineering), Southwest Jiaotong University, Chengdu 610031, China)
Abstract：Addressing the clinical challenge that current osteosarcoma treatment strategies struggle to simultaneously achieve tumor eradication and bone repair, this study designed a multifunctional nanomaterial to synchronously inhibiting osteosarcoma cells and protecting the proliferative activity of osteoblasts, thereby modulating homeostasis to facilitate bone defect repair. FeS2 nanoparticles with excellent peroxidase-like activity were loaded onto the surface of Ti3C2 MXenes, which possess near-infrared (NIR) photothermal response properties, constructing an FeS2/Ti3C2 composite carrier. The substrate was surface-functionalized using amino-polyethylene glycol-hydroxyl (NH2-PEG-OH). The active herbal component Puerarin was immobilized onto the composite carrier via dithiodiglycolic acid bridging technology, ultimately yielding the Puerarin@FeS2/Ti3C2 nanoplatform. Material characterization techniques including X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), Raman spectroscopy, and Fourier transform infrared spectroscopy (FT-IR) confirmed the successful preparation and composite structural features of the material. Cytotoxicity assays demonstrated that the material exhibited no significant toxicity to mouse fibroblast cells (L929) or macrophage cells (RAW264.7), with cell viability remaining over 80% after long-term co-culture. Under 1 064 nm NIR laser irradiation, the material exerts a photothermal-chemodynamic synergistic response. Compared with the single-stimulus groups, the composite material group exhibits enhanced inhibitory effects on osteosarcoma cell proliferation, with a UMR-106 cell viability of 54.3% after 2 days. At the same time, the introduction of Puerarin exerted a protective effect on BMSCs, showing no significant difference in the proliferation rate compared to the blank control group after 7 days. In conclusion, this work provided a novel strategy for developing integrated nanoplatforms for combined tumor therapy and bone defect repair.
青优, 李遂焰, 翁杰. Puerarin@FeS2/Ti3C2纳米平台构建及其骨肿瘤术后治疗功能研究[J]. 中国生物医学工程学报, 2026, 45(3): 352-361.
Qing You , Li Suiyan, Weng Jie. Fabrication of Puerarin@FeS2/Ti3C2 Nanoplatform for Postoperative Bone Tumor Therapy. Chinese Journal of Biomedical Engineering, 2026, 45(3): 352-361.
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