Ferric Chloride and Tannic Acid Treatment Enhance the Comprehensive Properties of Bovine Jugular Vein
Wang Aili1, Zhou Jianye1*, Wang De1, Zhou Qingliang2, Wang Yumiao1, Huo Meijun2, Wang Yibo1
1(State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100037, China) 2(Beijing Med Zenith Medical Scientific Co., Ltd, Beijing 101312, China)
Abstract：Glutaraldehyde (GA) cross-linked bovine jugular vein (BJV) is prone to calcification, which affects its wide clinical application. The addition of tannic acid (TA) treatment in combination with GA cross-linking has achieved excellent anti-calcification effect, however, with a deficiency of poor tissue compliance. As tannins can chelate with metal cations, this study used ferric ions (Fe3+) to interact with tannins to improve the comprehensive performance of TA treated BJV. In this study, the addition schemes of Fe3+ and reaction conditions such as pH were explored, the mechanical properties were examined by the uniaxial tensile test, the rat subcutaneous implantation model was used to determine the calcification level, and two-point bending method was used to test the flexibility. Vessels obtained from optimized treatment protocols were tested for biocompatibility. The results showed that under pH=8, the addition of Fe3+ before TA treatment improved the tissue compliance, and the flexibility was significantly improved compared with that of TA group, meanwhile, the mechanical strength was maintained. Calcium contents after subcutaneous implantation in rats were significantly lower than that in GA group [21d:(1.71±0.41) mg/g vs (38.12±7.40) mg/g, 60d:(2.73±1.13) mg/g vs(124.19±14.22) mg/g, P<0.05], and were equal to that of TA group (P>0.05). Moreover, the biocompatibility of theBJV met the requirements of implantable medical devices. This TA-Fe3+ treatment scheme is expected to be a new method for anti-calcification of BJV.
王爱莉, 周建业, 王德, 周庆亮, 王玉苗, 霍美俊, 汪一波. 铁离子-单宁酸处理增强牛颈静脉综合性能[J]. 中国生物医学工程学报, 2023, 42(3): 328-335.
Wang Aili, Zhou Jianye, Wang De, Zhou Qingliang, Wang Yumiao, Huo Meijun, Wang Yibo. Ferric Chloride and Tannic Acid Treatment Enhance the Comprehensive Properties of Bovine Jugular Vein. Chinese Journal of Biomedical Engineering, 2023, 42(3): 328-335.
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