Effect of Intranasal Mechanical Vibrational Stimulation on Resting-State EEG RelativePower and Effective Connectivity in Healthy Subjects
Yu Xiaoru1, Xu Wenlong1*, Xu Bingqiao1, Ge Qiaoling2
1(School of Information Engineering, China Jiliang University, Hangzhou 310018, China) 2(Department of Clinical Engineering, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310016, China)
Abstract：Intranasal mechanical vibrational stimulation (iMVS) is a new noninvasive nerve stimulation technique,and it gives rise to the adjustment of the intrinsic functional activity in the limbic system and restores the homeostasis in the autonomic nervous system. In this work, the neurophysiological mechanism of iMVS was explored by analyzing the effect of iMVS on the EEG relative power and EEG effective connectivity of healthy adults. The 22 healthy adults were recruited into the study and randomly divided into experimental group and control group. Each nasal cavity of 11 subjects in experimental group were performed with iMVS for 10 minutes, and the remained 11 subjects in the control group were performed with the sham stimulation. The resting-state EEG was recorded before and 30 minutes after iMVS. Welch transform was used to analyze the relative power; direct directed transfer function (dDTF) was used to obtain the effective connectivity; independent sample t-test, paired t-test and false discovery rate method were used forstatistical analysis. Results showed a significant improvement of alpha band relative power in experimental group (baseline: 51.57%±5.93%, 30 min: 57.33%±4.59%) and the relative power of C3, C4, T8, O1, O2 leads increased significantly in alpha band (P<0.05); beta band relative power was also enhanced significantly in experimental group (baseline: 7.28%±0.11%, 30 min: 8.36%±0.44%) and the relative power of C3, C4, T7, T8, O2 leads increased significantly in beta band (P<0.05). The dDTF value of alpha band was significantly enhanced in the experimental group (baseline: 0.052±0.0017, 30 min: 0.0592±0.0028), and the dDTF value of the directions, including F4 to F3, O2 to F3, C4 to F4, O2 to F4, F3 to C3, C4 to C3, F3 to T7, C4 to T8, O2 to O1, increased significantly in alpha band (P<0.05). There were no significant changes in the EEG relative power and EEG effective connectivity in the control group before and after stimulation. The intrinsic functional activity of limbic system was positively correlated with the EEG relative power of alpha and beta band as well as the EEG effective connectivity of alpha band. The results showed that iMVS enhanced the intrinsic functional activity of limbic system 30 minutes after the end of stimulation. The study explored the neurophysiological mechanism of iMVS, which improved the homeostasis of autonomic nervous system from the perspective of EEG analysis for the first time, and providedevidence for the use of EEG relative power and EEG effective connectivity as biomarkers of iMVS utility evaluation.
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