1(School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, China) 2(Zhejiang Provincial Collaborative Innovation Center of Agricultural Biological Resources Biochemical Manufacturing, Hangzhou 310023, China) 3(Zhejiang Provincial Key Laboratory of Chemistry and Biological Processing Technology of Farm Products, Hangzhou 310023, China)
Abstract：The main purpose of liver pathology research is to study the pathogenesis and drug development of fatty liver, hepatitis, liver fibrosis and liver cancer. At the same time, liver disease is complicated and difficult to treat and shows a tendency of disease progression. The development from fatty liver to cirrhosis and then to liver cancer requires a whole-process research model. At present, liver diseases are mainly studied through two-dimensional cell culture in vitro and animal models in vivo, but neither can fully reveal the pathogenesis of liver diseases and the effect of drug treatment. The development of liver organoids through three-dimensional culture technology makes the further study of liver diseases possible. In this paper, the advances in the field of liver organoids and their applications in biomedicine were reviewed. The key technologies including the selection of seed cells and the construction method of liver organoids were summarized, and their practical applications in building disease models, screening drugs and the detection of hepatotoxicity were introduced, which proved the importance of liver organoids.
景思佳, 郑慧琳, 张蕾. 肝脏类器官的研究进展[J]. 中国生物医学工程学报, 2021, 40(2): 237-246.
Jing Sijia, Zheng Huilin, Zhang Lei. Advances in the Development of Liver Organoids. Chinese Journal of Biomedical Engineering, 2021, 40(2): 237-246.
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